Cell Suspension Calculator

Calculate specimen volume, cells per tube, and target cell concentrations for flow cytometry and laboratory cell suspensions.

Calculates specimen volume to reach a target cell count per tube, or to prepare a suspension at a target cell concentration. Results update as you type.

Target Cells Per Tube Target Cell Concentration

Mode A: Target Cells Per Tube

Calculates specimen volume needed to deliver a target cell count per tube. Starting concentration and target cells per tube must refer to the same cell population.

Starting cell concentration

The concentration of the specimen as counted (e.g. on a hemocytometer or analyzer).

Desired cells per tube Total cells wanted in each tube, of the same population as above.

Number of tubes

Specimen per tube

—

Total specimen volume for all tubes

—

Show calculation

Volume per tube = Target cells per tube ÷ Cell concentration

Starting concentration converts to — cells/µL.

Volume per tube = — µL

Total specimen volume = Volume per tube × Number of tubes = — µL

Mode B: Target Cell Concentration

Calculates specimen and diluent volumes to prepare a suspension at a target concentration, using C1 × V1 = C2 × V2.

Concentration unit applies to both C1 and C2 below

Starting cell concentration (C1)

Desired final cell concentration (C2)

Desired final suspension volume (V2)

Specimen volume (V1)

—

Diluent volume

—

Final prepared volume

—

Dilution factor

—

Show calculation

V1 = (C2 × V2) / C1, then Diluent = V2 − V1

V1 = —

Diluent = —

Dilution factor = C1 ÷ C2 = —

Notice: CellCounter's calculators are intended as calculation aids only. All inputs are processed locally in your browser — nothing is transmitted, stored, or shared. Results must be independently verified in accordance with your institution's SOPs prior to use in specimen preparation.